Buthionine sulfoximine inhibits cytopathic effects and apoptosis induced by infection with AIK-HDC strain of measles virus.

Buthionine Sulfoximine Inhibits Cytopathic Effects and Apoptosis Induced by Infection with AIK-HDC Strain of Measles Virus

Measles virus (MV) is a highly contagious agent which causes a major health problem in developing countries. We studied the effect of buthionine sulfoximine (BSO) on the replication of an AIK-HDC strain of MV and its induced apoptosis in Vero cell lines. Methods: In this study, toxicity of BSO on Vero cells was investigated first, resulted in determination of sub-lethal or non-toxic concentrati...

Construction of a Minigenome Rescue System for Measles Virus, AIK-c Strain

Background:In the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded RNA viruses from cDNA or viral minigenomes. This technique has been applied to study different steps in virus replication and virus-host interactions. Reverse genetics could also be implemented for design of new vaccines. The T7 RNA polymerase activity as well as virus (nucleocapsi...

Sequence Variation in Measles Virus F Gene of AIK-C Vaccines Strain after Passage in Vero Cells

Critical role of GSH in Sulfur Mustard-induced Oxidative Stress and Cytotoxicity in Human Skin Fibroblast Cell Line

In this study the role of glutathione (GSH) in sulfur mustard -induced oxidative stress and cytotoxicity, in human skin fibroblast cell line (HF2FF) was evaluated. Sulfur mustard-induced superoxide radical and hydrogen peroxide formation were evaluated by determination of superoxide dismutase and catalase activity in cell lysate. The cytotoxicity of sulfur mustard was estimated by lactate dehyd...

A comparative field trial of five measles vaccines produced in human diploid cell, MRC-5.

Five batches of experimental measles vaccines were prepared with Biken-CAM, Schwarz, Sugiyama, Leningrade-16 and AIK-C strains in MRC-5 HDC. The rct 40, T50 or neurovirulence for baby hamsters of these viruses adapted to HOC were not significantly changed but the neurovirulence of the Sugiyama virus for day-old hamsters was increased after passages in HOC. Whith the exception of the AIK-C strai...